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Part:BBa_K1486021:Design

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-15)


Arabinose promoter + rLuc[1] + rLuc[2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

When designing the part, we took care of adding a stop codon at the end of the N terminal part of the luciferase and a start codon before the C terminus part. Between the two parts of the split is a short random sequence followed by a second RBS.

Source

The part has been made by PCR amplification of the plasmid prLuc from Waldor Laboratory [http://waldorlab.bwh.harvard.edu/] used in this paper [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0043175].

References

S.K. Hatzios, S. Ringgaard, B. M. Davis, M. K. Waldor (2012, August 15). Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition Plos One.